Curcumin inhibits growth, induces G1 arrest and apoptosis on human prostatic stromal cells by regulating Bcl-2/Bax.
- Author:
Zhi-qiang CHEN
1
;
Xiao JIE
;
Zeng-nan MO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Curcumin; pharmacology; Flow Cytometry; G1 Phase; drug effects; Humans; In Situ Nick-End Labeling; Male; Prostate; cytology; Reverse Transcriptase Polymerase Chain Reaction; Stromal Cells; drug effects; metabolism; Tumor Cells, Cultured; bcl-2-Associated X Protein; metabolism
- From: China Journal of Chinese Materia Medica 2008;33(16):2022-2025
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of curcumin on apoptosis of human prostatic stromal cells.
METHODDifferent concentrations of curcumin were added into culture media system to induce apoptosis of human prostatic stromal cells. The apoptosis was detected by MTT assay, flow cytometry, RT-PCR and TUNEL method.
RESULTCurcumin at concentrations of 10-40 micromol x L(-1) could inhibit the proliferation of human prostatic stromal cells in a doseand time-dependent manner (P < 0.05). Characteristic apoptosis were confirmed by TUNEL RT-PCR manifest downregulation of Bcl-2/Bax (P < 0.05). Cell cycle was arrested into G1 phase by curcumin.
CONCLUSIONCurcumin can induce apoptosis in human prostatic stromal cells by down-regulation of Bcl-2/Bax.
