Effects of puerarin on ADRP gene expression in fatty tissue of type 2 diabetes mellitus rats.
- Author:
Wei SUN
1
;
Xue-zhi ZHENG
;
Qiu-ling XU
;
Hong NIAN
;
Gui-lian LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; drug effects; metabolism; Animals; Blood Glucose; drug effects; Diabetes Mellitus, Experimental; drug therapy; genetics; Female; Gene Expression Regulation; drug effects; In Vitro Techniques; Isoflavones; pharmacology; Male; Membrane Proteins; genetics; Perilipin-2; Rats; Rats, Wistar; Reverse Transcriptase Polymerase Chain Reaction; Vasodilator Agents; pharmacology
- From: China Journal of Chinese Materia Medica 2008;33(16):2026-2060
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of puerarin on ADRP gene mRNA expression in fatty tissue of type 2 diabetes mellitus rats (T2DM).
METHODWiastar rats of T2DM model were made by feeding with high glucose and fat diet and injecting with small dose of streptozocin (25 mg x kg(-1)). 40 model rats were randomly divided into model control group and three puerarin groups (40, 80, 160 mg x kg(-1)), another 10 rats were selected as normal control group. FBG and FINS were measured to calculate IR after rats were injected consecutively for 6 weeks. The level of ADRP gene mRNA in fatty tissue was determined by RT-PCR after rats were injected eight weeks.
RESULTCompared with model control group, high and middle dosage of puerarin can decreased ADRP gene mRNA expression in fatty tissue obviously, FBG, IR level in each puerarin group and FINS in high and middle dosage puerarin groups decreased obviously.
CONCLUSIONPuerarin can decrease the blood glucose level of T2DM by downregulating ADRP mRNA expression and depressing the insulin resistance.
