Effects of exogenous connective tissue growth factor on collagen III synthesis of human renal tubular epithelial cells.
- Author:
Ai-Qing ZHANG
1
;
Wei-Hua GAN
;
Gui-Xia DING
;
Jing GONG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Collagen Type III; biosynthesis; genetics; Connective Tissue Growth Factor; Epithelial Cells; drug effects; metabolism; Humans; Immediate-Early Proteins; pharmacology; Immunohistochemistry; Intercellular Signaling Peptides and Proteins; pharmacology; Kidney Tubules; drug effects; metabolism; RNA, Messenger; analysis
- From: Chinese Journal of Contemporary Pediatrics 2008;10(2):188-190
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the role of exogenous connective tissue growth factor (CTGF) in the collagen III synthesis of human renal tubular epithelial cell line HK2 in vitro.
METHODSCultured HK2 cells were randomly assigned to three groups: placebo-control, low-dose CTGF-treated (2.5 ng/mL) and high-dose CTGF-treated groups (20 ng/mL). Cell morphological changes were observed under an inverted microscope. Collagen III alpha mRNA expression was detected using RT-PCR. Immunohistochemistry staining was used to assess the levels of intracellular collagen III alpha protein.
RESULTSAfter 48 hrs of low- or high- dose CTGF treatment, the appearances of HK2 cells were changed from oval to fusiform. High-dose CTGF treatment increased collagen III alpha mRNA expression (0.4461+/-0.0274 vs 0.2999+/-0.0115; P<0.05) as well as the protein expression of collagen III alpha (0.4075+/-0.0071 vs 0.3503+/-0.0136; P<0.05) compared with the placebo-control group.
CONCLUSIONSCTGF can induce morphological changes of human renal tubular epithelial cells in vitro. High concentration of CTGF may increase the synthesis of collagen III alpha.
