Scanning of c-kit gene mutations in acute myeloid leukemias using high-resolution melting analysis.
- Author:
Dong-ming YAO
1
;
Jun QIAN
;
Jiang LIN
;
Qin CHEN
;
Gao-fei XIAO
;
Ya-li WANG
;
Zhen QIAN
;
Run-bi JI
;
Yun LI
;
Jing YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; DNA Mutational Analysis; methods; Exons; Female; Humans; Leukemia, Myeloid, Acute; genetics; Male; Middle Aged; Mutation; Precursor Cell Lymphoblastic Leukemia-Lymphoma; genetics; Proto-Oncogene Proteins c-kit; genetics; Young Adult
- From: Chinese Journal of Hematology 2011;32(1):21-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the common mutations (D816V and N822K) of c-kit gene in acute myeloid leukemia (AML) using high-resolution melting analysis (HRM).
METHODSHRM analysis was established to screen c-kit mutations in PCR products of c-kit exon 17 in 21 AML patients with t(8;21). PCR products were sequenced to confirm the mutation.
RESULTSHRM analysis identified an aberrant melting curve in 6 cases (28.6%), which were confirmed by direct DNA sequencing as one D816V mutation and five N822K mutation.
CONCLUSIONHRM analysis is a convenient, rapid, specific and high-throughput technique for scanning c-kit gene mutation in AML.
