Effect of regulation of Y-box protein 1 by RNA interference on the doxorubicin induced mdr1 gene expression in K562 cells.
- Author:
Hui-Ling SHEN
1
;
Wen-Lin XU
;
Qiao-Yun CHEN
;
Fa-Chun WANG
;
Xia FEI
Author Information
- Publication Type:Journal Article
- MeSH: ATP Binding Cassette Transporter, Sub-Family B; ATP-Binding Cassette, Sub-Family B, Member 1; genetics; Doxorubicin; pharmacology; Drug Resistance, Neoplasm; drug effects; genetics; Gene Expression; drug effects; Gene Silencing; Humans; K562 Cells; Protein Transport; RNA Interference; RNA, Small Interfering; Y-Box-Binding Protein 1; genetics
- From: Chinese Journal of Hematology 2011;32(6):383-387
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of YB-1 on the transcription of induced mdr1 gene expression in K562 cells.
METHODSK562 cells were treated with doxorubicin (DOX) at different concentrations and times. Expression of mdr1 and YB-1 genes was examined by RT-PCR and P-glycoprotein (P-gp) by flow cytometry. Cyto/nuclear protein was extracted for YB-1 detection by Western blotting. The expression of YB-1 gene in K562 cells was inhibited by YB-1 gene specific RNA interference (RNAi), then the expression of mdr1 and P-gp in YB-1 gene silenced cells treated with DOX was detected.
RESULTSThe mdr1 gene as well as its corresponding protein P-gp was highly expressed in DOX exposed K562 cells. DOX up-regulated the expression of YB-1 gene, and promoted YB-1 protein nuclear translocation. On YB-1 gene silenced, the expressions of mdr1 gene and P-gp were obviously down-regulated in DOX treated K562 cells.
CONCLUSIONDoxorubicin can induce the expression of mdr1 gene in K562 cells, which may result from the transcription of mdr1 gene by activated YB-1.
