A preliminary study on the influence of human plasma exosomes-like vesicles on macrophage Wnt5A-Ca²+ pathway.
- Author:
Dan LI
1
;
Ya-Na REN
;
Jie YANG
;
Yi-Ming YANG
;
Chun-Yan LI
;
Ru-Feng XIE
;
Hua-Hua FAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Calcium; metabolism; Calcium Signaling; Exosomes; Female; Humans; Macrophage Activation; Macrophages; metabolism; Middle Aged; Proto-Oncogene Proteins; metabolism; Wnt Proteins; metabolism; Wnt-5a Protein; Young Adult
- From: Chinese Journal of Hematology 2011;32(6):404-407
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influence of human plasma exosomes-like vesicles on the regulatory function of macrophages.
METHODSThe exosomes-like vesicles were purified from healthy donors plasma with a series of high-speed centrifugation and ultrafiltration. Macrophages were derived from cultured human blood monocytes. The molecular markers of macrophages were assayed by FACS. After cultured with exosomes-like vesicles, the changes of macrophages cytoplasma Ca(2+), and related genes and proteins were assayed by FACS, RT-PCR and Western Blot, respectively.
RESULTSAfter cultured with exosomes-like vesicles, mean fluorescent intensity (MFI) of macrophages cytoplasma Ca(2+) was increased. The vesicles enhanced macrophages to express cytokines genes, the expression of IL-1β and TNF-α genes being increased by 0.85 and 1.69 times respectively at 2 h, and that of IL-6 gene 3.7 times compared with the control at 8 h. However, the vesicles inhibited the expression of macrophages IL-10 gene, had no influence on the Frizzled5 receptor expression and could induce CaMKII phosphorylation.
CONCLUSIONSExosomes-like vesicles can up-regulat macrophages expression of inflammatory cytokines genes, and increase the secretion of inflammatory cytokines by activating the Wnt5A-Ca(2+) signaling pathway.