Co-expression of sCD40LIg and CTLA4Ig mediated by adenovirus prolonged mouse skin allograft survival.
- Author:
Zhao-lun LI
1
;
Pu-xun TIAN
;
Wu-jun XUE
;
Jun WU
Author Information
- Publication Type:Journal Article
- MeSH: Abatacept; Adenoviridae; genetics; Animals; Cytopathogenic Effect, Viral; Graft Survival; immunology; Immunoconjugates; genetics; immunology; metabolism; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; RNA, Messenger; biosynthesis; genetics; Recombinant Fusion Proteins; biosynthesis; genetics; immunology; Reverse Transcriptase Polymerase Chain Reaction; Skin Transplantation; immunology; methods; Transfection
- From: Journal of Zhejiang University. Science. B 2006;7(6):436-444
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the role of simultaneous blockade of CD40/CD40L and B7/CD28 pathways in the immune tolerance via co-expression of sCD40LIg and CTLA4Ig mediated by replication-defective adenovirus.
METHODSAd-sCD40LIg-IRES(2)-CTLA4Ig, replication-defective adenovirus co-expressing sCD40LIg and CTLA4Ig, was constructed and identified. The co-expression of sCD40LIg and CTLA4Ig was evaluated with confocal laser scanning microscope and Western blotting. Skin transplantations of C57BL/6 to BALB/c mice were performed. PBS, Ad-Shuttle-CMV and Ad-sCD40LIg-IRES(2)-CTLA4Ig were administered. Skin graft survival was monitored and the mRNA expression of both genes was evaluated in the skin allografts.
RESULTSAd-sCD40LIg-IRES(2)-CTLA4Ig was constructed successfully and identified. The co-expression of sCD40LIg and CTLA4Ig was identified with confocal laser scanning microscopy and Western blotting. Compared to the skin graft mean survival time (MST) of non-treated group ((5.75+/-0.71) d) or Ad-Shuttle-CMV-treated group ((5.50+/-0.53) d), the skin graft MST was dramatically prolonged in the Ad-sCD40LIg-IRES(2)-CTLA4Ig-treated group ((16.38+/-1.19) d, P<0.001). The mRNA expression of both genes was detected.
CONCLUSIONAd-sCD40LIg-IRES(2)-CTLA4Ig, a replication-defective adenovirus carrying genes encoding sCD40LIg and CTLA4Ig, was constructed. Simultaneous blockade of CD40/CD40L and B7/CD28 costimulatory pathway mediated by replication-defective adenovirus significantly prolonged skin allograft survival in mice.
