Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients.
- Author:
Dong XU
1
;
Xu-fen LI
;
Shu ZHENG
;
Wen-zhi JIANG
Author Information
1. Cancer Institute, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. honokiol@gmail.com
- Publication Type:Journal Article
- MeSH:
Adult;
Aged;
Aged, 80 and over;
Biomarkers, Tumor;
biosynthesis;
blood;
genetics;
Carcinoembryonic Antigen;
biosynthesis;
blood;
genetics;
Carcinoma;
blood;
genetics;
Colorectal Neoplasms;
blood;
genetics;
Female;
Humans;
Keratin-20;
Keratins;
biosynthesis;
blood;
genetics;
Male;
Middle Aged;
RNA, Messenger;
blood;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Sensitivity and Specificity
- From:
Journal of Zhejiang University. Science. B
2006;7(6):445-451
- CountryChina
- Language:English
-
Abstract:
This study is aimed at establishing a sensitive approach to detect disseminated tumor cells in peripheral blood and evaluate its clinical significance. A total of 198 blood samples including 168 from colorectal carcinoma (CRC) patients and 30 from healthy volunteers were examined by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) to evaluate the expression of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and cytokeratin 19 (CK19) mRNA. CEA mRNA was detected in 35.8% of patients and 3.3% of controls, CK20 mRNA in 28.3% of patients and 6.7% of controls, and CK19 mRNA in 41.9% of patients and 3.3% of controls. CEA and CK20 mRNA positive ratio increased with the advancing Dukes stages, but there was no significant difference in positive ratio between any two stages (P>0.05). Also, relatively high positive ratio of CEA, CK20 and CK19 mRNA expression was observed in some CRC patients with earlier Dukes stages. A higher positive ratio was obtained when two or three detection markers were combined compared to a single marker. Our study indicates that quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood is a valuable tool for monitoring early stage dissemination of CRC cells in blood circulation.
