Antitumor activities of D-glucosamine and its derivatives.
- Author:
Li ZHANG
1
;
Wan-shun LIU
;
Bao-qin HAN
;
Yan-fei PENG
;
Dong-feng WANG
Author Information
1. College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. qdzhangli@ouc.edu.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Antineoplastic Agents;
therapeutic use;
Apoptosis;
drug effects;
Cell Cycle;
drug effects;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
DNA;
metabolism;
DNA Fragmentation;
drug effects;
Glucosamine;
analogs & derivatives;
therapeutic use;
Humans;
Liver Neoplasms;
drug therapy;
pathology;
Male;
Mice;
Sarcoma 180;
drug therapy
- From:
Journal of Zhejiang University. Science. B
2006;7(8):608-614
- CountryChina
- Language:English
-
Abstract:
The growth inhibitory effects of D-glucosamine hydrochloride (GlcNH(2).HCl), D-glucosamine (GlcNH(2)) and N-acetyl glucosamine (NAG) on human hepatoma SMMC-7721 cells in vitro were investigated. The results showed that GlcNH(2).HCl and GlcNH(2) resulted in a concentration-dependent reduction in hepatoma cell growth as measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. This effect was accompanied by a marked increase in the proportion of S cells as analyzed by flow cytometry. In addition, human hepatoma SMMC-7721 cells treated with GlcNH(2).HCl resulted in the induction of apoptosis as assayed qualitatively by agarose gel electrophoresis. NAG could not inhibit the proliferation of SMMC-7721 cells. GlcNH(2).HCl exhibited antitumor activity against Sarcoma 180 in Kunming mice at dosage of 125-500 mg/kg, dose of 250 mg/kg being the best. GlcNH(2).HCl at dose of 250 mg/kg could enhance significantly the thymus index, and spleen index and could promote T lymphocyte proliferation induced by ConA. The antitumor effect of GlcNH(2).HCl is probably host-mediated and cytocidal.
