Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum.
- Author:
Yi-xiong ZHENG
1
;
Jochen RINGE
;
Zhong LIANG
;
Alexander LOCH
;
Li CHEN
;
Michael SITTINGER
Author Information
1. Department of Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. zyx_xxn@hotmail.com
- Publication Type:Journal Article
- MeSH:
Biocompatible Materials;
Bone Development;
Cell Culture Techniques;
methods;
Cell Differentiation;
Cell Survival;
Cells, Cultured;
Collagen;
chemistry;
Humans;
Lactic Acid;
chemistry;
Microscopy, Fluorescence;
Models, Statistical;
Osteogenesis;
Periosteum;
metabolism;
Polyglycolic Acid;
chemistry;
Polymers;
chemistry;
Stem Cells;
cytology;
Tissue Engineering
- From:
Journal of Zhejiang University. Science. B
2006;7(10):817-824
- CountryChina
- Language:English
-
Abstract:
The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic medium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo.
