Research advances of the influence factors of high level expression of recombinant protein in Pichia pastoris.
- Author:
Qing-Hua WANG
;
Li-Li GAO
;
Hui-Chao LIANG
;
Ting GONG
;
Jin-Ling YANG
;
Ping ZHU
- Publication Type:Journal Article
- MeSH:
Fermentation;
Gene Dosage;
Glycosylation;
Molecular Chaperones;
Pichia;
metabolism;
Promoter Regions, Genetic;
Protein Sorting Signals;
Recombinant Proteins;
biosynthesis
- From:
Acta Pharmaceutica Sinica
2014;49(12):1644-1649
- CountryChina
- Language:Chinese
-
Abstract:
Pichia pastoris is one of the most important systems used in the field of molecular biology for the expression of recombinant proteins. The system has advantages of high expression, high stability, high secretion, easy high-density fermentation and low cost. Many factors affect the expression of recombinant protein, such as gene copy number, codon usage preference, type of promoter, molecular chaperones, glycosylation, signal peptide and fermentation process. In this review, research advances of the above aspects are summarized, which lay a foundation for improving the expression of recombinant proteins in P. pastoris.
