Effects of crocetin on VCAM-1 expression in human umbilical vein endothelial cells and monocyte-endothelial cell adhesion.
- Author:
Shu-guo ZHENG
;
Meng-qiu ZHAO
;
You-nan REN
;
Jie-ren YANG
;
Zhi-yu QIAN
- Publication Type:Journal Article
- MeSH:
Angiotensin II;
metabolism;
Antioxidants;
pharmacology;
Carotenoids;
pharmacology;
Cell Adhesion;
drug effects;
Human Umbilical Vein Endothelial Cells;
cytology;
drug effects;
metabolism;
Humans;
Monocytes;
cytology;
NF-kappa B;
metabolism;
Reactive Oxygen Species;
metabolism;
Vascular Cell Adhesion Molecule-1;
metabolism
- From:
Acta Pharmaceutica Sinica
2015;50(1):34-38
- CountryChina
- Language:Chinese
-
Abstract:
Crocetin, a naturally occurring carotenoid, possesses antioxidant and antiatherosclerotic properties, of which the underlying mechanism remains unclear. In the present study, we examined the effects of crocetin (0.1, 1, 10 μmol·L(-1)) on angiotensin II (Ang II, 0.1 μmol·L(-1)) induced expression of vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) and monocyte-endothelial cell adhesion. The effects of crocetin on the activation of nuclear factor kappa B (NF-κB) and intracellular reactive oxygen species (ROS) were also observed. The results demonstrated that crocetin notably suppressed Ang II induced NF-κB activation (P<0.01) and VCAM-1 expression (P<0.05, P<0.01) in HUVECs, accompanied by a markedly reduced monocyte-endothelial cell adhesion (P<0.05, P<0.01). In addition, preincubation with crocetin resulted in a significant enhancement of cellular antioxidant capacity (P<0.05, P<0.01), while Ang II induced intracellular ROS decreased markedly (P<0.05, P<0.01). These results indicated that crocetin was capable of suppressing Ang II induced VCAM-1 expression and monocyte-endothelial cell adhesion by suppression of NF-κB activation, which might be derived from the enhancement of antioxidant capacity and subsequent reduction of intracellular ROS.
