Effect of ethyl gallate on invasion abilities and its mechanism of breast cancer MDA-MB-231 cells.
- Author:
Hong-xia CUI
;
Ming WANG
;
Jia-xin YUAN
;
Ji-cheng LIU
- Publication Type:Journal Article
- MeSH:
Breast Neoplasms;
pathology;
Cell Adhesion;
drug effects;
Cell Line, Tumor;
Cell Movement;
drug effects;
Gallic Acid;
analogs & derivatives;
pharmacology;
Humans;
Matrix Metalloproteinase 2;
metabolism;
Matrix Metalloproteinase 9;
metabolism;
NF-kappa B;
metabolism;
Phosphorylation;
Proto-Oncogene Proteins c-akt;
metabolism;
RNA, Messenger;
Signal Transduction
- From:
Acta Pharmaceutica Sinica
2015;50(1):45-49
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the effect of ethyl gallate on invasion capabilities and its mechanism of breast cancer MDA-MB-231 cells. Using cell adhesion and transwell assay, separately, the effects of ethyl gallate on the invasion of MDA-MB-231 cells were measured. The Akt-NF-κB signal pathway protein expressions were analyzed with Western blot. Also, the mRNA levels of MMP-9 and MMP-2 were analyzed by RT-PCR. Ethyl gallate inhibited the abilities of motility, adhesion and invasion of breast cancer MDA-MB-231 cells in vitro (P<0.05), inhibited the mRNA levels of MMP-9, MMP-2, phosphorylation of AKt and protein expression of NF-κB. It is concluded that ethyl gallate can inhibit the abilities of invasion of breast cancer in vitro by inhibiting the mRNA levels of MMP-9/MMP-2, phosphorylation of Akt and protein expression of NF-κB.
