Design and synthesis of photoaffinity biotin labelled 2'-O-propargyl-guanosine.
- Author:
Lu-xin NA
;
Xin LIU
;
Zhuo-ming MENG
;
Zhu GUAN
;
Li-he ZHANG
;
Zhen-jun YANG
- Publication Type:Journal Article
- MeSH:
Biotin;
chemistry;
Click Chemistry;
Guanosine;
chemical synthesis;
chemistry;
Ligands;
Photoaffinity Labels
- From:
Acta Pharmaceutica Sinica
2015;50(1):59-63
- CountryChina
- Language:Chinese
-
Abstract:
Photoaffinity labeling is widely applied to demonstrate targets of small molecule ligands. In this paper, biotin photoaffinity labeled molecule with propargyl group 1 has been designed and synthesized, followed it's labeling of N2-acetyl-2'-O-propargyl guanosine 9 by "click chemistry". This technology presents delight development potential in labeling of second messenger cyclic nucleotide, antisense oligonucleotide or siRNA.
