Chemical constituents of Taxus chinensis var. mairei cell cultures.
- Author:
Xiang-yang BAI
;
Jian-ming LÜ
;
Yan-ying ZHOU
;
Zi-rong ZHU
;
Ren-wang JIANG
;
Wei ZHANG
- Publication Type:Journal Article
- MeSH:
Alkenes;
analysis;
Cell Culture Techniques;
Cells, Cultured;
Diterpenes;
analysis;
Molecular Structure;
Paclitaxel;
analysis;
Sitosterols;
analysis;
Taxoids;
analysis;
Taxus;
chemistry
- From:
Acta Pharmaceutica Sinica
2015;50(1):70-74
- CountryChina
- Language:Chinese
-
Abstract:
The chemical constituents of Taxus chinensis var. mairei cell cultures were investigated by chromatographic methods, including silica gel column chromatography, Sephadex LH-20 and preparative HPLC. Thirteen compounds were isolated from the 80% ethanol extract of cultured cells and their structures were elucidated by spectral data and physicochemical properties, which were identified as 2α,4α,7β,9α,10β-pentaacetoxy-14β-hydroxytax-11-ene (1), 2α,4α,7β,9α,10β-pentaacetoxytax-11-ene (2), 1β-deoxybaccatin VI (3), 2α-acetoxytaxusin (4), taxuyunnanine C (5), yunnanxane (6), 2α,5α,10β-triacetoxy-14β-propionyloxy-4 (20), 11-taxadiene (7), 2α,5α,10β-triacetoxy-14β-isobutyryloxy-4 (20), 11-taxadiene (8), 2α,5α,10β-triacetoxy-14β-(2'-methyl)butyryloxy-4 (20), 11-taxadiene (9), 13-dehydroxylbaccatin III (10), 13-dehydroxy-10-deacetylbaccatin III (11), paclitaxel (12) and (13) β-sitosterol. Among them, compound 1 is a new compound, and compounds 2, 4, 10 and 11 are isolated from the cell culture of Taxus chinensis var. mairei for the first time.
