Characterization and comparison of interferon reference standards using UPLC-MS.
- Author:
Lei TAO
;
De-ning PEI
;
Chun-mei HAN
;
Wei CHEN
;
Chun-ming RAO
;
Jun-zhi WANG
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Chromatography, High Pressure Liquid;
methods;
Interferons;
standards;
Mass Spectrometry;
methods;
Molecular Weight;
Oxidation-Reduction;
Peptide Mapping;
Protein Processing, Post-Translational;
Reference Standards
- From:
Acta Pharmaceutica Sinica
2015;50(1):75-80
- CountryChina
- Language:Chinese
-
Abstract:
The study aims to characterize and compare interferon reference standards from 5 manufacturers. By testing molecular mass and trypsin-digested peptide mass mapping, the amino acid sequence was verified and post-translational modifications such as disulfide bond were identified. Results show that the molecular mass and amino acid sequence were consistent with theory; the disulfide bonds of 4 lots of interferon were Cys1-Cys98/Cys29-Cys138, 1 lot was Cys29-Cys139/Cys86-Cys99; N-terminal "+Met", acetyl N-terminal and Met oxidation were identified in part of the sample. UPLC-MS can be used to characterize and compare interferon reference standards from different manufacturers.
