Pandanus tectorius derived caffeoylquinic acids inhibit lipid accumulation in HepG2 hepatoma cells through regulation of gene expression involved in lipid metabolism.
- Author:
Chong-ming WU
;
Hong LUAN
;
Shuai WANG
;
Xiao-po ZHANG
;
Hai-tao LIU
;
Peng GUO
- Publication Type:Journal Article
- MeSH:
Carcinoma, Hepatocellular;
metabolism;
China;
Cholesterol;
metabolism;
Gene Expression Regulation;
Hep G2 Cells;
Humans;
Lipid Metabolism;
Liver Neoplasms;
metabolism;
Oleic Acid;
Pandanaceae;
chemistry;
Quinic Acid;
analogs & derivatives;
chemistry;
Sterol Regulatory Element Binding Protein 1;
Triglycerides;
metabolism
- From:
Acta Pharmaceutica Sinica
2015;50(3):278-283
- CountryChina
- Language:Chinese
-
Abstract:
The fruit of Pandanus tectorius (PTF) has a long history of use as a folk medicine to treat hyperlipidemia in Hainan province, South China. Our previous studies have shown that the n-butanol extract of PTF is rich in caffeoylquinic acids and has an adequate therapeutic effect on dyslipidemic animals induced by high-fat diet. In this work, seven caffeoylquinic acids isolated from PTF were screened for the lipid-lowering activity in HepG2 hepatoma cells. Oil-Red O staining, microscopy and intracellular triglyceride (TG) and total cholesterol (TC) quantification showed that 3-O-caffeoylquinic acid (3-CQA), 3, 5-di-O-caffeoylquinic acid (3,5-CQA), and 3,4,5-tri-O-caffeoylquinic acid (3,4,5-CQA) significantly inhibited lipid accumulation induced by oleic acid and decreased intracellular levels of TC and TG in a dose-dependent manner. These three caffeoylquinic acids showed no significant cytotoxicity at concentrations of 1 -50 μmol x L(-1) as determined by MTT assay. Realtime quantitative PCR revealed that 3-CQA and 3, 5-CQA significantly increased the expression of lipid oxidation-related genes PPARα, CPT-1 and ACOX1 while 3-CQA, 3, 5-CQA and 3,4,5-CQA decreased the expression of lipogenic genes SREBP-1c, SREBP-2, HMGR, ACC, FAS. Overall, 3-CQA, 3, 5-CQA and 3, 4, 5-CQA may be the principal hypolipidemic components in PTF which can decrease intracellular lipid accumulation through up-regulating the expression of lipid oxidative genes and down-regulating the expression of lipogenic genes.
