Comparison of three different methods in transfection of plasmid pGPU6/GFP/Neo to chicken embryo fibroblasts.
- Author:
Xing HE
1
;
Hua LI
;
Yan SHAO
;
Ying HU
;
Zichun GU
;
Li CHEN
;
Jingjing MA
;
Zhiyong LAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Chick Embryo; Fibroblasts; cytology; Green Fluorescent Proteins; genetics; Plasmids; genetics; Transfection; methods
- From: Journal of Zhejiang University. Medical sciences 2013;42(6):660-665
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare three different methods in transfection of plasmid pGPU6/GFP/Neo into chicken embryo fibroblast.
METHODSDifferent doses (1.25 μl,2 μl,2.5 μl) of Lipofec-tamin2000, Gbfectene-Elite and HilyMax were used to transfect 1 μg plasmid pGPU6/GFP/Neo. The transfection efficiency was observed by the fluorescence and the cell viabilities were measured.
RESULTSThe transfection efficiency of HilyMax was significant higher than that of Lipofectamin2000 and Gbfectene-Elite (86.85%±2.32% compared with 48.33%±3.24% and compared with 37.35%±5.41%; F=18.882, P<0.05). The transfection efficiency of 2.5 μl HilyMax was highest(90.53%±1.15%). The cell viability of Lipofectamin2000 was significantly lower than that of HilyMax and Gbfectene-Elite(65.76%±5.78% compared with 89.54%±0.86% and compared with 82.45%±3.56%;F=90.676, P<0.05).
CONCLUSIONHilyMax has the highest transfection efficiency and the lowest cell toxicity in transfection of plasmid pGPU6/GFP/Neo into chicken embryo fibroblast, with the optimal ratio of 2.5 μl HilyMax:1 μg plasmid.
