Inhibition of osthole for resorption of rats femur tissue in vitro.
- Author:
Jian ZHOU
;
Xue-mei REN
;
Xiao-ni MA
;
Yu-hai GAO
;
Li-juan YAN
;
Wen-gui SHI
;
Ke-ming CHEN
- Publication Type:Journal Article
- MeSH: Acid Phosphatase; metabolism; Animals; Bone Resorption; prevention & control; Coumarins; pharmacology; Estradiol; pharmacology; Femur; drug effects; Glucose; analysis; Lactic Acid; analysis; Male; Rats; Rats, Sprague-Dawley
- From: China Journal of Orthopaedics and Traumatology 2015;28(9):832-837
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate osthole effect on femoral tissue resorption activity of rat in vitro.
METHODSSix SD rats weighted (80 ± 5) g were used to isolate and culture femoral tissue (diaphyses and metaphysis) in vitro. The cultured tissue were devided into control group, estradiol group and osthole group. The femoral tissue was treated with final concentration of 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol culture in vitro at 48 hours after cultured. Tartrate-resistant acid phosphatase (StrACP) activity, glucose and Lactic acid content, StrACP, MCSF (Macrophage colony stimulating factor) and CTSK (Cathepsin K) mRNA was detected by Real-Time RT-PCR were detected.
RESULTSConcetration of Alkaline phosphatase activity were 2226 and 2498 in 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol respectively. As compared with control group, the activity of StrACP of 1 x 10(-5) mol/L osthole and 1 x 10(-8) mol/L estradiol were inhibited at 6, 9, 12 days (P < 0.05); under treatment of in l x 10(-5) mol/L osthole, the content of Lactic acid were increased and the content of glucose were decreased at 3, 6, 9 days (P < 0.05); StrACP, MCSF and CTSK mRNA expression level were inhibited at 6, 9 days (P < 0.05).
CONCLUSIONOsthole can inhibit bone resorption and raise the level of nutrition metabolism of femurs tissue.