Application of spectral karyotyping to cytogenetic analysis in acute myeloid leukemia.
- Author:
Bo GUO
1
;
Hong-Li ZHU
;
Su-Xia LI
;
Xiao-Ping HAN
;
Jing-Fen SUN
;
Li-Li WANG
;
Wen-Rong HUANG
;
Wan-Ming DA
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Cytogenetic Analysis; Female; Humans; Karyotyping; Leukemia, Myeloid, Acute; genetics; Male; Middle Aged; Spectral Karyotyping; Young Adult
- From: Chinese Journal of Hematology 2011;32(7):454-457
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the value of spectral karyotyping (SKY) in cytogenetic analysis of acute myeloid leukemias (AML).
METHODSNine AML patients were analyzed by R-banding and SKY. MLL, PML-RARalpha, AML1-ETO fusion genes were detected by dual fusion- fluorescence in situ hybridization (D-FISH).
RESULTSAll 9 samples were successfully hybridized. SKY identified structural aberrations including 9q -, t(15;17) and ins(10;17) (q22;p11p12) ; and some numeral abnormalities. The results of SKY confirmed those of R-band karyotyping and D-FISH; with more accurate localization.
CONCLUSIONSKY appears to be fairly stable, accurate and sensitive, for AML cytogenetic study.
