The effects and mechanisms of erythropoietin on hepcidin of human monocytes.
- Author:
Xiao HAN
1
;
Dao-Bin ZHOU
;
Cai-Min XU
;
Yang YANG
;
Ming-Hui DUAN
;
Xuan WANG
;
Jie-Ping ZHANG
;
Yong-Qiang ZHAO
;
Ti SHEN
;
Yong-Ji WU
Author Information
- Publication Type:Journal Article
- MeSH: Antimicrobial Cationic Peptides; metabolism; Cells, Cultured; Erythropoietin; pharmacology; Hepcidins; Humans; Interleukin-6; pharmacology; Lipopolysaccharides; pharmacology; Monocytes; drug effects; metabolism; Signal Transduction
- From: Chinese Journal of Hematology 2011;32(7):468-472
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the in vitro effect of erythropoietin (EPO) on hepcidin of monocytes and its molecular mechanisms.
METHODSHepcidin and signaling molecules including C/EBPalpha, Smad1/5/8, p-Smad1/5/8 and p-STAT3 were detected by real time PCR and Western blot. THP-1 monocytes were stimulated by interleukin-6 (IL-6) or lipopolysaccharide (LPS). EPO receptor (EPOR) antibody was added to observe its antagonistic effect on EPO and impact on the signaling proteins.
RESULTSEPO suppressed mRNA expression of THP-1 hepcidin of monocytes induced by 20 ng/ml IL-6 or 1 microg/ml LPS in both dose and time dependent manner. The most decrease of hepcidin expression was observed at 2 IU/ml EPO for 6 hours. EPO also down-regulated hepcidin protein induced by 20 ng/ml IL-6. At 2 IU/ml EPO for 6 hours hepcidin protein was down-regulated, as was C/EBPalpha, p-Smad1/5/8 and p-STAT3. Antibody to EPOR antagonized the down-regulation of EPO on hepcidin and signaling proteins.
CONCLUSIONSMonocytes hepcidin can be reduced by EPO when stimulated by IL-6 or LPS. The mechanism of which may be at least in part, via suppression of C/EBPalpha, p-Smad1/5/8 and p-STAT3 signaling.
