The role of Th17 cells in immune thrombocytopenia mice models.
- Author:
Ting-ting WANG
1
;
Hui-yuan LI
;
Zhao WANG
;
Ze-ping ZHOU
;
Li FU
;
Na WEI
;
Da-yong HUANG
;
Ren-chi YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Female; Mice; Mice, Inbred BALB C; Th17 Cells; immunology; Thrombocytopenia; immunology
- From: Chinese Journal of Hematology 2011;32(9):592-596
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of Th17 cells in immune thrombocytopenia (ITP) mice model.
METHODITP was induced by daily intraperitoneal injection of anti-platelet membrane CD41 antibody (MWReg30) into BALB/c mice, the mRNA expressions of Th17 cell associated transcription factors and cytokines in peripheral blood and spleen mononuclear cells were measured by real-time PCR, and the proportion of Th17 cells by FCM analysis.
RESULTSThe percentage of Th17 cell was significantly elevated in ITP mice both in splenocyte and peripheral blood as compared with that in normal controls (P<0.01). ITP mice had elevated mRNA expressions of IL-17F, IL-17A and IL-6 in splenocyte (P<0.05), and of IL-21 in peripheral blood (P<0.05). There was a positive correlation between IL-17A and IL-17F (r = 0.934, P = 0.000), and between IL-17A/IL-17F and IL-6 (r = 0.598, P = 0.001; r = 0. 619, P = 0.000).
CONCLUSIONSTh17 cell might play an important role in the pathogenesis of ITP, at least involving in the clearance of platelets.
