EPOR and TPOR expressions on CD34+ CD59- and CD34+ CD59+ bone marrow cells from patients with paroxysmal nocturnal hemoglobinuria.
- Author:
Dan WANG
1
;
Rong FU
;
Er-Bao RUAN
;
Wen QU
;
Yong LIANG
;
Hua-Quan WANG
;
Jun WANG
;
Li-Juan LI
;
Hui LIU
;
Hong-Lei WANG
;
Tian ZHANG
;
Hong LIU
;
Yu-Hong WU
;
Li-Min XING
;
Guo-Jin WANG
;
Xiao-Ming WANG
;
Jia SONG
;
Jing GUAN
;
Zong-Hong SHA
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Bone Marrow Cells; metabolism; CD59 Antigens; metabolism; Case-Control Studies; Cells, Cultured; Female; Flow Cytometry; Hemoglobinuria, Paroxysmal; metabolism; Humans; Male; Middle Aged; Receptors, Erythropoietin; metabolism; Receptors, Thrombopoietin; metabolism; Young Adult
- From: Chinese Journal of Hematology 2011;32(8):543-547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expressions of erythropoietin receptor (EPOR) and thrombopoietin receptor (TPOR) on CD34+ CD59- and CD34+ CD59+ bone marrow (BM) cells from patients with paroxysmal nocturnal hemoglobinuria (PNH).
METHODS(1) The expressions of EPOR and TPOR on CD34+ CD59- and CD34+ CD59- BM cells from 26 PNH patients and 16 normal controls were examined by flow cytometry (FCM). (2) The mRNA expression of the EPOR and the TPOR in BM mononuclear cells (BMMNC) from 25 PNH patients and 13 normal controls were examined by RT-PCR.
RESULTS(1) The percentage of EPOR positive cells in PNH CD34+ CD59+ BMMNC [(30.67 +/- 18.30)%] was significantly higher than that in PNH CD34+ CD59- BMMNC [(8.05 +/- 3.51)%] (P < 0.01) and than that in control CD34+ CD59+ BMMNC [(8.24 +/- 6.51)%] (P < 0.01), but there was no obvious difference between the CD34+ CD59-BMMNC in PNH and CD34+ CD59+ BMMNC in control. (2) The percentage of TPOR positive cells in PNH CD34+ CD59+ BMMNC [(28.15 +/- 17.75)%] was significantly higher than that in PNH CD34+ CD59-BMMNC [(15.65 +/- 14.45)%] (P < 0.05) and than that in control CD34+ CD59+ BMMNC [(10.77 +/- .39)%] (P < 0.01), but there was no obvious difference between the CD34+ CD59- BMMNC in PNH and CD34+ CD59+ BMMNC in control. (3) There was no statistic difference in EPOR mRNA and TPOR mRNA expressions in BMMNCs between PNH patients group [(0.41 +/- 0.37) and (0.32 +/- 0.19), respectively] and control group [(0.47 +/- 0.33) and (0.40 +/- 0.29), respectively].
CONCLUSIONThe expression of EPOR and TPOR of PNH patients on BM CD34+ CD59+ cells are significantly higher than those on BM CD34+ CD59- cells. The difference may be due to abnormal transcription of both receptor coding genes.
