Dexamethasone reduces IL-17 and Tim-3 expression in BALF of asthmatic mice.
10.1007/s11596-013-1145-4
- Author:
Xiao-xia LU
1
;
Karen S MCCOY
;
Wei-kun HU
;
Jia-li XU
;
Hai-qin WANG
;
Peng CHEN
;
He-bin CHEN
Author Information
1. Department of Pulmonary Medicine, Wuhan Women and Children Health Care Center, Wuhan, 430030, China, lusi74@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Asthma;
drug therapy;
genetics;
metabolism;
Bronchoalveolar Lavage Fluid;
chemistry;
Dexamethasone;
pharmacology;
Female;
Gene Expression;
drug effects;
genetics;
Hepatitis A Virus Cellular Receptor 2;
Interleukin-17;
genetics;
metabolism;
Mice;
Mice, Inbred BALB C;
Receptors, Virus;
genetics;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(4):479-484
- CountryChina
- Language:English
-
Abstract:
This study investigated the expression of interleukin-17 (IL-17) and T cell immunoglobulin mucin and domain-containing molecule-3 (Tim-3) in bronchoalveolar lavage fluid (BALF) of asthmatic mice and the effect of dexamethasone (DEX) on these factors. Thirty-six mice were randomly divided into three groups: normal group, asthmatic group and DEX group. The mouse model of asthma was established by sensitization with ovalbumin in both the asthmatic and DEX groups. The levels of IL-6, IL-10, IL-17 and TGF-β were measured in BALF by enzyme-linked immunesorbent assay (ELISA). The mRNA expression level of Tim-3 was detected by reverse transcription polymerase chain reaction (RT-PCR). The ratio of Tim-3+CD4+ cells to total CD4+ cells in BALF was determined by flow cytometry. Differential inflammatory cells in BALF were detected. The correlations among IL-17, IL-6, IL-10, Tim-3 and inflammatory cells were analyzed. The results showed that the levels of IL-17, IL-6 and Tim-3 were substantially increased and the IL-10 level decreased in BALF in the asthmatic mice, which was significantly reversed by DEX treatment. IL-17 expression was positively correlated with IL-6 and Tim-3 expression and the number of inflammatory cells but negatively with IL-10 expression. These results indicate that the increased expression of IL-17 and Tim-3 in BALF may be implicated in the occurrence and development of asthmatic inflammation; the mechanism by which DEX suppresses asthmatic airway inflammation involves down-regulation of IL-17 and Tim-3 levels.
