Inhibitory effect of dexamethasone on expression of cysteine-rich 61 protein in airway epithelial cells of allergic mouse models.
10.1007/s11596-013-1170-3
- Author:
Yong CAO
1
;
Hui-Long CHEN
1
;
Sheng CHENG
1
;
Jun-Gang XIE
1
;
Wei-Ning XIONG
1
;
Yong-Jian XU
1
;
Hui-Juan FANG
2
Author Information
1. Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
2. Department of Respiratory and Critical Care Medicine, Tongji Hospital, Key Laboratory of Pulmonary Diseases of the Ministry of Health of China, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. fanghuijuan@yahoo.com.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Inflammatory Agents;
administration & dosage;
pharmacology;
Asthma;
chemically induced;
drug therapy;
metabolism;
Bronchoalveolar Lavage Fluid;
chemistry;
cytology;
Chemokines, CC;
metabolism;
Cysteine-Rich Protein 61;
biosynthesis;
Dexamethasone;
administration & dosage;
pharmacology;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells;
drug effects;
metabolism;
pathology;
Female;
Immunohistochemistry;
Injections, Intraperitoneal;
Leukocyte Count;
Lung;
metabolism;
pathology;
Mice;
Mice, Inbred BALB C;
Neutrophils;
drug effects;
pathology;
Ovalbumin
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(5):628-631
- CountryChina
- Language:English
-
Abstract:
In order to study whether cysteine-rich 61 protein (cyr61) is involved in the pathogenesis of asthma and its relation to airway inflammation, the effect of dexamethasone (Dxm) on the expression of cyr61 in the lung tissues of asthmatic mice was investigated. Forty BALB/c mice were divided into asthma group (n=15), control group (n=10) and Dxm group (n=15). The asthma group was sensitized and challenged by ovalbumin (OVA). The mice in Dxm group were intraperitoneally administered with Dxm after OVA challenge. The expression of cyr61 in the lung tissues was detected by using immunohistochemistry, and that of eotaxin protein in the bronchoalveolar lavage fluid (BALF) by using enzyme-linked immunosorbent assay (ELISA). The number of inflammatory cells in BALF was also analyzed. The results showed that the cyr61 expression was highest in asthma group (P<0.05), followed by Dxm group (P<0.05) and control group. The cyr61 had a positive correlation with the total nucleated cells (r=0.867, P<0.05), especially eosinophils (r=0.856, P<0.05), and eotaxin level (r=0.983, P<0.05) in the BALF. Our findings suggested that cyr61 is expressed in airway epithelial cells and has a positive correlation with eotaxin and number of airway infiltrating eosinophils.
