Downregulation of p38 MAPK involved in inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin.
10.1007/s11596-013-1178-8
- Author:
Ju-Mei XIA
1
;
Jun ZHANG
2
;
Wen-Xiang ZHOU
3
;
Xiao-Cheng LIU
1
;
Min HAN
4
Author Information
1. Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
2. Department of Nephrology, the First Affiliated Hospital of Xiamen University, Xiamen, 361003, China.
3. Department of Nephrology, Wuhan First Hospital, Wuhan, 430022, China.
4. Department of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. minhan@tjh.tjmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Anti-Inflammatory Agents, Non-Steroidal;
pharmacology;
Blotting, Western;
Cell Proliferation;
drug effects;
Cells, Cultured;
Curcumin;
pharmacology;
Cyclooxygenase 2;
genetics;
metabolism;
Dose-Response Relationship, Drug;
Down-Regulation;
Enzyme Inhibitors;
pharmacology;
Extracellular Matrix;
drug effects;
metabolism;
Gene Expression;
drug effects;
Imidazoles;
pharmacology;
Lipoproteins, LDL;
pharmacology;
Matrix Metalloproteinase 2;
genetics;
metabolism;
Mesangial Cells;
drug effects;
metabolism;
Pyridines;
pharmacology;
Rats;
Rats, Sprague-Dawley;
Reactive Oxygen Species;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
p38 Mitogen-Activated Protein Kinases;
antagonists & inhibitors;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(5):666-671
- CountryChina
- Language:English
-
Abstract:
Curcumin, as a main pharmacological component in the traditional Chinese medicine-turmeric, has shown anti-inflammatory, anti-oxidation, anti-tumor and anti-fibrotic effects. This study aimed to investigate the possible underlying signaling pathway which was involved in the inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin. Rat mesangial cells in vitro were incubated with low-density lipoprotein (LDL) and different concentrations of curcumin (0, 6.25, 12.5, 25.0 μmol/L) or p38 MAPK inhibitor, SB203580 (10 μmol/L). Under LDL incubation, mesangial cells proliferated, the expression of MMP-2 mRNA and protein was decreased, the expression of COX-2 mRNA and protein was increased, reactive oxygen species (ROS) generation was increased and p38 MAPK was activated significantly (P<0.05). When LDL-induced cells were treated with curcumin in the concentration of 12.5 or 25.0 μmol/L, LDL-induced proliferation of mesangial cells was suppressed, the expression of MMP-2 mRNA and protein increased, the expression of COX-2 mRNA and protein downregulated, the production of ROS inhibited and p38 MAPK inactivated (P<0.05). In conclusion, curcumin can inhibit the LDL-induced proliferation of mesangial cells and up-regulate the expression of MMP-2, which may be related with the inhibitory effect of curcumin on COX-2 expression, ROS production and p38 MAPK.
