Assessment of the number and function of macrophages in the placenta of gestational diabetes mellitus patients.
10.1007/s11596-013-1187-7
- Author:
Jun YU
1
;
Yong ZHOU
1
;
Juan GUI
1
;
Ai-Zhen LI
1
;
Xiao-Ling SU
1
;
Ling FENG
2
Author Information
1. Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
2. Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. fltj007@163.com.
- Publication Type:Journal Article
- MeSH:
Adult;
Antigens, CD;
Antigens, Differentiation, Myelomonocytic;
Cell Count;
Cytokines;
genetics;
immunology;
metabolism;
Diabetes, Gestational;
genetics;
immunology;
metabolism;
Female;
Humans;
Immunohistochemistry;
Inflammation Mediators;
immunology;
metabolism;
Interleukin-6;
genetics;
immunology;
metabolism;
Lipopolysaccharide Receptors;
genetics;
metabolism;
Macrophages;
immunology;
metabolism;
pathology;
Placenta;
immunology;
metabolism;
Pregnancy;
Reverse Transcriptase Polymerase Chain Reaction;
Tumor Necrosis Factor-alpha;
genetics;
immunology;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(5):725-729
- CountryChina
- Language:English
-
Abstract:
In order to assess the number and function of macrophages in the placenta of pregnancy complicated with gestational diabetes mellitus (GDM) as well as those of normal pregnancies, placenta samples were collected from 15 GDM patients (GDM group) and 10 normal pregnant women (control group). The expression levels of macrophage markers (CD68/CD14) and inflammatory cytokines (IL-6/TNF-α) in placenta were detected using immunohistochemistry and PCR. The results showed that the number of CD68+ or CD14+ cells in the GMD group was remarkably higher than that in the control group (P<0.05), indicating that the number of macrophages in the GDM group was significantly greater than that in the control group. The mRNA expression levels of CD68+, IL-6 and TNF-α were higher in the GMD group than in the control group. In conclusion, more macrophages accumulate in placenta of pregnancy complicated with GDM, and the expression levels of pro-inflammation factors are also increased in GDM pregnancies, suggesting that macrophages and inflammatory mediators (IL-6 and TNF-α) may play an important role in GDM.
