Optimization of parameters for preparation of docetaxel-loaded PLGA nanoparticles by nanoprecipitation method.
10.1007/s11596-013-1192-x
- Author:
Wei SHI
1
;
Zhan-Jie ZHANG
1
;
Yin YUAN
1
;
En-Ming XING
1
;
You QIN
1
;
Zhen-Jun PENG
2
;
Zhi-Ping ZHANG
3
;
Kun-Yu YANG
1
Author Information
1. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
2. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. zhenjunp@sina.com.cn.
3. School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
- Publication Type:Journal Article
- MeSH:
Acetone;
chemistry;
Antineoplastic Agents;
chemistry;
pharmacokinetics;
Chromatography, High Pressure Liquid;
Drug Compounding;
methods;
Ethanol;
chemistry;
Fractional Precipitation;
methods;
Lactic Acid;
chemistry;
Microscopy, Electron, Scanning;
Nanoparticles;
chemistry;
ultrastructure;
Nanotechnology;
methods;
Particle Size;
Polyethylene Glycols;
chemistry;
Polyglycolic Acid;
chemistry;
Succinates;
chemistry;
Surface Properties;
Taxoids;
chemistry;
pharmacokinetics
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(5):754-758
- CountryChina
- Language:English
-
Abstract:
The purpose of this study was to develop docetaxel-poly (lactide-co-glycolide) (PLGA) loaded nanoparticles by using nanoprecipitation method and optimize the relative parameters to obtain nanoparticles with higher encapsulation efficiency and smaller size. The physicochemical characteristics of nanoparticles were studied. The optimized parameters were as follows: the oil phase was mixture of acetone and ethanol, concentration of tocopheryl polyethylene glycol succinate (TPGS) was 0.2%, the ratio of oil phase to water phase was 1:5, and the theoretical drug concentration was 5%. The optimized nanoparticles were spherical with size between 130 and 150 nm. The encapsulation efficiency was (40.83±2.1)%. The in vitro release exhibited biphasic pattern. The results indicate that docetaxel-PLGA nanoparticles were successfully fabricated and may be used as the novel vehicles for docetaxel, which would replace Taxotere® and play great roles in future.
