Suppression of low-dose hyper-radiosensitivity in human lung cancer cell line A549 by radiation-induced autophagy.
10.1007/s11596-013-1195-7
- Author:
Yan-Xia ZHAO
1
;
Chen CHENG
1
;
Fang ZHU
1
;
Hong-Ge WU
1
;
Jing-Hua REN
1
;
Wei-Hong CHEN
1
;
Jing CHENG
2
Author Information
1. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430023, China.
2. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430023, China. chenjin1118@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Adenine;
analogs & derivatives;
pharmacology;
Autophagy;
drug effects;
radiation effects;
Blotting, Western;
Cell Line, Tumor;
Cell Survival;
drug effects;
radiation effects;
Dose-Response Relationship, Radiation;
Flow Cytometry;
Green Fluorescent Proteins;
genetics;
metabolism;
Humans;
Lung Neoplasms;
genetics;
metabolism;
pathology;
Microscopy, Confocal;
Microscopy, Electron, Transmission;
Microtubule-Associated Proteins;
genetics;
metabolism;
Phagosomes;
drug effects;
radiation effects;
ultrastructure;
Radiation Tolerance;
drug effects;
radiation effects
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(5):770-774
- CountryChina
- Language:English
-
Abstract:
This study explored the role of radiation-induced autophagy in low-dose hyperradiosensitivity (HRS) in the human lung cancer cell line A549. A549 cells, either treated with an autophagic inhibitor 3-methyladenine (3-MA), or with a vehicle control, were irradiated at different low doses (≤0.5 Gy). The generation of autophagy was examined by laser scanning confocal microscopy. Western blotting was used to detect the expression of microtubule-associated protein l light chain 3B II (LC3B-II). Flow cytometry (FCM) and clonogenic assays were used to measure the fraction of surviving cells at the low irradiation doses. Our results showed that there was a greater inhibition of autophagic activity, but a higher degree of low-dose HRS in A549 cells treated with 3-MA than in control group. Our data demonstrated that radiation-induced autophagy is correlated with HRS in A549 cells, and is probably one of the mechanisms underlying HRS.
