Genotoxicity and reduced heat shock protein 70 in human airway smooth muscle cells exposed to cigarette smoke extract.
10.1007/s11596-013-1206-8
- Author:
Xiao-jie WU
1
;
Guo-xiong LUO
;
Xue ZENG
;
Li-Li LAN
;
Qin NING
;
Yong-jian XU
;
Jian-ping ZHAO
;
Jun-gang XIE
Author Information
1. Department of Respiratory Medicine and Critical Care Medicine, Huazhong University of Science and Technology, Wuhan, 430030, China, 463890611@qq.com.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
DNA Damage;
Deoxyguanosine;
analogs & derivatives;
metabolism;
HSP70 Heat-Shock Proteins;
genetics;
metabolism;
Humans;
Lung;
cytology;
drug effects;
Myocytes, Smooth Muscle;
drug effects;
metabolism;
Smoke;
adverse effects;
Tobacco;
toxicity;
Tumor Cells, Cultured
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2013;33(6):827-833
- CountryChina
- Language:English
-
Abstract:
Cigarette smoke is associated with the development of several diseases, such as chronic obstructive pulmonary disease (COPD). The purpose of this study was to investigate genotoxicity and heat shock protein 70 (Hsp70) in human airway smooth muscle cells (HASMCs) exposed to cigarette smoke extract (CSE). HASMCs was exposed to CSE with different doses for 24 h. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined by using HPLC-ECD, the DNA damage was analyzed by using comet assay, and apoptosis was examined by using Annexin-FITC/PI staining. The production of Hsp70 after CSE stimulation was tested. Results indicated that CSE significantly increased the level of 8-OHdG, DNA damage and cell apoptosis, and reduced the production of Hsp70. In particular, levels of Hsp70 were inversely correlated with 8-OHdG, DNA damage and cell apoptosis. It was concluded that cigarette smoke induced genotoxicity and decreased the production of cell protective protein Hsp70, which may contribute to the development of some airway diseases.
