Thymic stromal lmphopoietin pomotes macrophage-derived foam cell formation.
10.1007/s11596-014-1226-z
- Author:
Da-zhu LI
1
;
Bo-yuan WANG
;
Bao-jie YANG
;
Shao-lin HE
;
Jing LIN
;
Jiang-chuan DONG
;
Chun WU
;
Jun HU
Author Information
1. Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China, lidazhuhp@sohu.com.
- Publication Type:Journal Article
- MeSH:
ATP Binding Cassette Transporter 1;
genetics;
metabolism;
Animals;
Antibodies;
immunology;
pharmacology;
Blotting, Western;
CD36 Antigens;
genetics;
metabolism;
Cells, Cultured;
Cholesterol;
metabolism;
Cholesterol Esters;
metabolism;
Cytokines;
pharmacology;
Dose-Response Relationship, Drug;
Foam Cells;
cytology;
drug effects;
metabolism;
Gene Expression;
drug effects;
Immunoglobulins;
immunology;
metabolism;
Lipoproteins, LDL;
pharmacology;
Macrophages;
cytology;
drug effects;
metabolism;
Mice;
Mice, Inbred C57BL;
Receptors, Cytokine;
immunology;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Scavenger Receptors, Class A;
genetics;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(1):23-28
- CountryChina
- Language:English
-
Abstract:
The effect of thymic stromal lymphopoietin (TSLP) on macrophage-derived foam cell formation and the underlying mechanism were studied. Macrophages isolated from C57BL/6 mice were co-cultured in vitro with different concentrations of TSLP or TSLPR-antibody in the presence of oxidized low density lipoprotein (ox-LDL). The effects of TSLP on macrophage-derived foam cell formation were observed by using oil red O staining and intracellular lipid determination. The expression levels of foam cell scavenger receptors (CD36 and SRA) as well as ABCA1 and TSLPR were detected by using RT-PCR and Western blotting. As compared with the control group, TSLP treatment significantly promoted lipid accumulation in macrophages, significantly increased protein expression of CD36 and TSLPR in a dose-dependent manner, and significantly reduced the expression of ABCA1 protein in a dose-dependent manner. No significant differences were noted between the TSLPR-antibody group and the control group. TSLP may down-regulate the expression of cholesterol efflux receptor ABCA1 and up-regulate scavenger receptor expression via the TSLPR signaling pathway, thereby promoting macrophage-derived foam cell formation.
