Role of Wnt/β-catenin signaling pathway in the mechanism of calcification of aortic valve.
10.1007/s11596-014-1228-x
- Author:
Gang-jian GU
1
;
Tao CHEN
;
Hong-min ZHOU
;
Ke-xiong SUN
;
Jun LI
Author Information
1. Department of Cardiothoracic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China, gugangjian627@163.com.
- Publication Type:Journal Article
- MeSH:
Alkaline Phosphatase;
genetics;
metabolism;
Animals;
Aortic Valve;
metabolism;
pathology;
Aortic Valve Stenosis;
Blotting, Western;
Bone Morphogenetic Protein 2;
genetics;
metabolism;
Calcinosis;
Cell Differentiation;
drug effects;
genetics;
Cells, Cultured;
Gene Expression;
drug effects;
Glycogen Synthase Kinase 3;
genetics;
metabolism;
Lipoproteins, LDL;
pharmacology;
Osteoblasts;
drug effects;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Swine;
Wnt Signaling Pathway;
genetics;
physiology;
beta Catenin;
genetics;
metabolism
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(1):33-36
- CountryChina
- Language:English
-
Abstract:
Aortic valve calcification is a common disease in the elderly, but its cellular and molecular mechanisms are not clear. In order to verify the hypothesis that Wnt/β-catenin signaling pathway is involved in the process of calcification of aortic valve, porcine aortic valve interstitial cells (VICs) were isolated, cultured and stimulated with oxidized low density lipoprotein (ox-LDL) for 48 h to induce the differentiation of VICs into osteoblast-like cells. The key proteins and genes of Wnt/β-catenin signaling pathway, such as glycogen synthase kinase 3β (GSK-3β) and β-catenin, were detected by using Western blotting and real-time polymerase chain reaction (PCR). The results showed that the VICs managed to differentiate into osteoblast-like cells after the stimulation with ox-LDL and the levels of proteins and genes of GSK-3β and β-catenin were increased significantly in VICs after stimulation for 48 h (P<0.05). It is suggested that Wnt/β-catenin signaling pathway may play a key role in the differentiation of VICs into osteoblast-like cells and make great contribution to aortic valve calcification.
