Preparation of PLLA/bpV(pic) microspheres and their effect on nerve cells.
10.1007/s11596-014-1234-z
- Author:
Qiang LIN
1
;
Hai-yun CHEN
;
Hao-shen LI
;
Yang-ting CAI
Author Information
1. The Second Department of Orthopedic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510006, China, qiang_linm@163.com.
- Publication Type:Journal Article
- MeSH:
Animals;
Axons;
drug effects;
physiology;
Cells, Cultured;
Delayed-Action Preparations;
chemistry;
pharmacokinetics;
pharmacology;
Drug Compounding;
Female;
Ganglia, Spinal;
drug effects;
metabolism;
physiology;
Immunohistochemistry;
Lactic Acid;
chemistry;
pharmacokinetics;
pharmacology;
Microscopy, Electron;
Microspheres;
Neural Stem Cells;
drug effects;
physiology;
Neurofilament Proteins;
metabolism;
Neurons;
drug effects;
metabolism;
Organometallic Compounds;
chemistry;
pharmacokinetics;
pharmacology;
Polyesters;
Polymers;
chemistry;
pharmacokinetics;
pharmacology;
Pregnancy;
Rats
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2014;34(1):76-80
- CountryChina
- Language:English
-
Abstract:
In this study, we prepared PLLA/bpV(pic) microspheres, a bpV(pic) controlled release system and examined their ability to protect nerve cells and promote axonal growth. PLLA microspheres were prepared by employing the o/w single emulsification-evaporation technique. Neural stem cells and dorsal root ganglia were divided into 3 groups in terms of the treatment they received: a routine medium group (cultured in DMEM), a PLLA microsphere group (DMEM containing PLLA microspheres alone) and a PLLA/bpV(pic) group [DMEM containing PLLA/bpV(pic) microspheres]. The effects of PLLA/bpV(pic) microspheres were evaluated by the live-dead test and measurement of axonal length. Our results showed that PLLA/bpV(pic) granulation rate was (88.2±5.6)%; particle size was (16.8±3.1)%, drug loading was (4.05±0.3)%; encapsulation efficiency was (48.5±1.8)%. The release time lasted for 30 days. In PLLA/bpV(pic) microsphere group, the cell survival rate was (95.2 ±4.77)%, and the length of dorsal root ganglion (DRG) was 718±95 μm, which were all significantly greater than those in ordinary routine medium group and PLLA microsphere group. This preliminary test results showed the PLLA/bpV(pic) microspheres were successfully prepared and they could promote the survival and growth of neural cells in DRG.
