Effect of Candida albicans on Intestinal Ischemia-reperfusion Injury in Rats.

Lei Yan; Chun-rong WU; Chen Wang; Chun-hui Yang; Guang-zhi Tong; Jian-guo Tang

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Effect of Candida albicans on Intestinal Ischemia-reperfusion Injury in Rats.

Author: Lei YAN ¹ ; Chun-Rong WU ² ; Chen WANG ³ ; Chun-Hui YANG ² ; Guang-Zhi TONG ⁴ ; Jian-Guo TANG ²
Author Information

1. Department of Emergency Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
2. Department of Trauma, Emergency and Critical Care Medicine, Shanghai Fifth People's Hospital, Fudan University, Shanghai 200240, China.
3. Department of Respiratory, Taizhou Municipal Hospital, Taizhou, Zhejiang 318000, China.
4. Division of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agriculture Sciences, Shanghai 200240, China.
Publication Type:Journal Article
MeSH: Amine Oxidase (Copper-Containing); metabolism; Animals; Anti-Bacterial Agents; pharmacology; Candida albicans; drug effects; pathogenicity; Cefoperazone; pharmacology; Enzyme-Linked Immunosorbent Assay; Female; Interleukin-1beta; metabolism; Interleukin-6; metabolism; Intestines; drug effects; immunology; metabolism; Rats; Rats, Wistar; Reperfusion Injury; immunology; metabolism; microbiology
From: Chinese Medical Journal 2016;129(14):1711-1718
CountryChina
Language:English
Abstract:
BACKGROUNDInflammation is supposed to play a key role in the pathophysiological processes of intestinal ischemia-reperfusion injury (IIRI), and Candida albicans in human gut commonly elevates inflammatory cytokines in intestinal mucosa. This study aimed to explore the effect of C. albicans on IIRI.
METHODSFifty female Wistar rats were divided into five groups according to the status of C. albicans infection and IIRI operation: group blank and sham; group blank and IIRI; group cefoperazone plus IIRI; group C. albicans plus cefoperazone and IIRI (CCI); and group C. albicans plus cefoperazone and sham. The levels of inflammatory factors tumor necrosis factor (TNF)-μ, interleukin (IL)-6, IL-1β, and diamine oxidase (DAO) measured by enzyme-linked immunosorbent assay were used to evaluate the inflammation reactivity as well as the integrity of small intestine. Histological scores were used to assess the mucosal damage, and the C. albicans blood translocation was detected to judge the permeability of intestinal mucosal barrier.
RESULTSThe levels of inflammatory factors TNF-μ, IL-6, and IL-1β in serum and intestine were higher in rats undergone both C. albicans infection and IIRI operation compared with rats in other groups. The levels of DAO (serum: 44.13 ± 4.30 pg/ml, intestine: 346.21 ± 37.03 pg/g) and Chiu scores (3.41 ± 1.09) which reflected intestinal mucosal disruption were highest in group CCI after the operation. The number of C. albicans translocated into blood was most in group CCI ([33.80 ± 6.60] ×102 colony forming unit (CFU)/ml).
CONCLUSIONIntestinal C. albicans infection worsened the IIRI-induced disruption of intestinal mucosal barrier and facilitated the subsequent C. albicans translocation and dissemination.