Matrix metalloproteinase-9/tissue inhibitor of matrix metalloproteinase-1 in induced sputum of bronchial asthmatics.
- Author:
Cheol Woo KIM
1
;
Hae Jin KIM
;
Jung Won PARK
;
Chein Soo HONG
Author Information
1. Department of Internal Medicine, Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Bronchial asthma;
sputum;
MMP-9;
TIMP-1
- MeSH:
Airway Remodeling;
Asthma;
Cell Movement;
Collagen;
Eosinophils;
Extracellular Matrix;
Homeostasis;
Humans;
Interleukin-8;
Matrix Metalloproteinase 1*;
Matrix Metalloproteinases;
Metalloproteases;
Molar;
Neutrophils;
Sputum*;
Tissue Inhibitor of Metalloproteinase-1
- From:Journal of Asthma, Allergy and Clinical Immunology
2000;20(6):916-926
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Bronchial asthma is a chronic inflammatory disease of the airways characterized by inflammatory cell infiltrations, which require extracellular matrix (ECM) breakdown and inflammatory cell migration. Airway remodeling with ECM deposition is another characteristic of asthma and reflect imbalance of collagen homeostasis. Collagen homeostasis is regulated by balance of metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). OBJECTIVE: We performed this study to evaluate the clinical significance and the role of MMPs and TIMPs in induced sputum of patients with symptomatic asthma. METHODS: We measured the concentrations of MMP-9 and its tissue inhibitor, TIMP-1, in induced sputum of 16 symptomatic asthmatics, 6 active smokers, and 5 healthy control subjects. RESULTS: Concentrations of MMP-9 and TIMP-1 were greater in patients with asthma than in control subjects. The molar ratio between MMP-9 and TIMP-1 was significantly lower in asthmatics than in control subjects (p<0.05). In asthmatics, MMP-9 concentrations were correlated with the number of total inflammatory cells, neutrophils and eosinophils (Rho=0.618, Rho=0.545 and Rho=0.384, p<0.01, p<0.01 and p=0.058, respectively). The concentrations of MMP-9 was negatively correlated with FEV1 (Rho=-0.467, p<0.05) and positively correlated with the levels of sputum ECP and IL-8 (Rho=0.595 and Rho=0.769, p<0.01 and p<0.01, respectively). CONCLUSION: MMP-9 may be involved in active inflammatory processes in symptomatic chronic airway disease, and the lower ratio of MMP-9/TIMP-1 in asthmatics suggests that MMP-9/TIMP-1 imbalance may be involved in airway remodeling.
