Characterization of an unusual variant mRNA of human lysosomal alpha-mannosidase.
- Author:
Huaichang SUN
1
;
John H WOLFE
Author Information
1. Laboratory of Pathology and Center for Comparative Medical Genetics, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104, USA.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
- Keywords:
lysosomal a-mannosidase;
intron sequences;
mRNA;
splice signal sequence
- MeSH:
*Alternative Splicing;
Base Sequence;
DNA, Complementary/genetics;
Exons;
Hela Cells;
Human;
Lysosomes/*enzymology;
Mannosidases/*genetics;
Molecular Sequence Data;
RNA, Messenger/*genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Support, U.S. Gov't, P.H.S.
- From:Experimental & Molecular Medicine
2000;32(4):187-192
- CountryRepublic of Korea
- Language:English
-
Abstract:
Lysosomal alpha-mannosidase (EC 3.2.1.24) is an exoglycosidase in the glycoprotein degradation pathway and is encoded by a 3.0 kb cDNA. A 2.3 kb cDNA from a minor species of HeLa cell mRNA was discovered by RT-PCR cloning. Southern blotting and PCR analysis of the HeLa cell genomic DNA showed that the 2.3 kb message was encoded by the lysosomal alpha-mannosidase gene. Sequence comparison of the cDNA with the corresponding genomic DNA indicated that the 2.3 kb message was generated by an unusual intra-exonic joining event.
