Effect and mechanism of Andrographitis Herba on human CD4+ T cell promoters CXCR4 and CCR5.
- Author:
Long FENG
1
;
Guoqiang ZHAO
;
Yunyun MA
;
Min LI
;
Jing MA
;
Jing JIN
;
Ying CUI
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Andrographis; chemistry; Animals; CD4-Positive T-Lymphocytes; drug effects; metabolism; Cell Line; Down-Regulation; drug effects; Drugs, Chinese Herbal; pharmacology; Female; Humans; Male; Promoter Regions, Genetic; drug effects; Rats; Rats, Wistar; Receptors, CCR5; genetics; metabolism; Receptors, CXCR4; genetics; metabolism; Young Adult
- From: China Journal of Chinese Materia Medica 2011;36(21):3012-3017
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEUtilizing a gene reporter technique to study the effects of Andrographitis Herba on human CXCR4 and CCR5 promoters.
METHODInhibition of CXCR4 and CCR5 on T cells of healthy volunteers was analyzed by RT PCR, Western blot and flow cytometry. The human CXCR4 and CCR5 promoters driving a luciferase reporter in vectors pGLA. 17-CXCR4 and pGLA. 17-CCR5 were transfected into H9 stem cells. G418 was used for selecting stable cell lines. Rat sera thus medicated was collected and added to the transfected H9 cells, in which the expression of CXCR4 and CCR5 promoters was detected.
RESULTThey showed that the mRNA and protein expression levels of CXCR4 and CCR5 in human CD4+ T cells decreased significantly after taking Andrographitis Herba (P<0.05). Furthermore human CXCR4 and CCR5 promoter activity was downregulated significantly by sera from rats medicated with Andrographitis Herba.
CONCLUSIONAndrographitis Herba may have the effect of down-regulating CXCR4 and CCR5 promoters. It provides a feasible experimental platform for screening herbal medicine as the treatment of HIV/AIDS.
