Environments of ethidium binding to allosteric Dna: II. Accessibility, mobility and mode of binding.
- Author:
Dong Chul SUH
1
Author Information
1. Department of Biochemistry, School of Medicine, Pochon CHA University, Kyungki-Do, Korea. DCSuh@hanmail.net
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
allosteric DNA;
fluorescence energy transfer;
fluorescence polarization;
fluorescence quenching;
mode of binding
- MeSH:
Allosteric Regulation;
Circular Dichroism;
DNA/*chemistry/*metabolism;
Energy Transfer;
Ethidium/*metabolism;
Exodeoxyribonucleases/metabolism;
Ligands;
Motion;
Nucleic Acid Conformation;
Oligodeoxyribonucleotides/*metabolism
- From:Experimental & Molecular Medicine
2000;32(4):204-209
- CountryRepublic of Korea
- Language:English
-
Abstract:
DNA binding compounds were previously shown to bind to the right-handed DNA forms and hybrid B-Z forms in a highly cooperative manner and indicate that structural specificity plays a key role in a ligand binding to DNA. In this study, the modes of binding and structural specificity of agents to unusual DNA are examined by a variety of fluorescence techniques (intensity, polarization and quenching, etc.) to explore a reliable method to detect the association environment of ligands to deoxyoligonucleotides initially containing a B-Z junction between the left-handed Z-DNA and right-handed B-DNA. The results of fluorescence energy transfer measurement demonstrated that the ligand molecules bind to the allosterically converted DNA structures by intercalation. In the absence of high-resolution structural data, this fluorescence energy transfer measurement allowed reliable measures and infer the binding environment of ligands to the allosteric DNA structures.
