Determination of loganin in Pterocephalus hookeri by HPLC.
- Author:
De TAN
1
;
Rui GU
;
Yi ZHANG
;
Xianli MENG
;
Xianrong LAI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Iridoids; analysis; Medicine, Tibetan Traditional; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(24):3472-3474
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of loganin in Pterocephalus hookeri.
METHODThe analysis was performed on a Welchrom C18 column (4.6 mm x 250 mm, 5 microm) with acetonitrile-water (12:88) as the the mobile phase at a flow rate of 1 mL x min(-1) and the detection wavelength was 240 nm.
RESULTThe peak areas and injection amounts of loganin had a good linear relationship at the range of 0.06-1.2 microg. The average recovery was 97.0%, and the RSD was 1.4%.
CONCLUSIONThe method is simple, accurate, and can be used for its quality control.
