Effect of berberine on expression of transforming growth factor-beta1 and type IV collagen proteins in mesangial cells of diabetic rats with nephropathy.
- Author:
Liqin TANG
1
;
Fei LV
;
Sheng LIU
;
Shantang ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Berberine; pharmacology; Collagen Type IV; genetics; Diabetes Mellitus, Experimental; metabolism; Diabetic Nephropathies; metabolism; Gene Expression Regulation; drug effects; Male; Mesangial Cells; drug effects; metabolism; Rats; Rats, Wistar; Streptozocin; Transforming Growth Factor beta1; genetics
- From: China Journal of Chinese Materia Medica 2011;36(24):3494-3497
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect and mechanism of berberine on diabetic nephropathy in experimental rats.
METHODThe rat model of diabetic nephropathy was induced by injection of streptozocin (STZ). The rats were divided into 6 groups: control group, model group, 3 berberine treatment groups and Xiaoke Wan (XKW) treatment group. The fasting blood glucose (FBG), kidney weight/body weight (KW/BW), blood urea nitrogen (BUN), creatinine (Cr) and urinary protein (Upro) were tested 8 weeks later. The expression of transforming growth factor-beta1 (TGF-beta1) and type IV collagen (IV-C) proteins in renal tissue of diabetic rats with nephropathy were observed by optical micrography.
RESULTBerberine could reduce the levels of FBG, KW/BW, BUN, Cr, Upro and the expression of TGF-beta1 and IV-C proteins in renal tissue of diabetic rats with nephropathy.
CONCLUSIONBerberine may protect renal function and slow down the progression of diabetic nephropathy in rats by suppressing the expression of TGF-beta1 and IV-C proteins in renal tissue.
