Studies on intestinal absorption of alkaloids in Coptis chinensis by in situ single-pass perfused rat intestinal model.
- Author:
Ying CHEN
1
;
Qing YANG
;
Lijuan ZOU
;
Yujie LI
;
Yiwei WANG
;
Xiaogang WENG
;
Wei MEN
;
Xiaoxin ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; pharmacokinetics; Animals; Berberine; pharmacokinetics; Berberine Alkaloids; pharmacokinetics; Coptis; Intestinal Absorption; Male; Perfusion; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2011;36(24):3523-3527
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the absorption characteristics of alkaloids in Coptis chinensis in rat intestinal.
METHODIn situ single-pass perfused rat intestinal model was used and the concentrations of berberine and palmatine were determined by HPLC, the drug absorption rate constant (K(a)) and apparent absorption coefficient (P(app)) were tested to evaluate their absorption characteristics.
RESULTThe absorption of berberine and palmatine in different regions of rat intestinals was significantly different. From bigness to smallness, the P(app) value of berberine at 50 mg x L(-1) were ileum, duodenum, jejunum and colon, of palmatine at 50 mg x L(-1) were ileum, colon, jejunum and duodenum. The concentration of berberine and palmatine had distinctive effect on the K(a) value of berberine and palmatine were ordered from larger to little as in Wujiwan compatibility, Coptis chinensis extractive and single compound.
CONCLUSIONThe berberine and palmatine were absroped at all regions of rat intestinal. The absorption characteristics of 2 alkaloids were not passive diffusion. The other ingredients in Wujiwan compatibility and Coptis chinensis extractive could promote the absorption of alkaloids.
