Effects of pseudolaric acid B on apoptosis of bladder cancer cell 5637.
- Author:
Gengqing QU
1
;
Yuwen SHENG
;
Bo CHEN
;
Ben LIU
;
Lichun YU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Caspase 3; analysis; Cell Cycle; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Diterpenes; pharmacology; Drugs, Chinese Herbal; pharmacology; Humans; Inhibitor of Apoptosis Proteins; analysis; Urinary Bladder Neoplasms; drug therapy; pathology
- From: China Journal of Chinese Materia Medica 2011;36(24):3535-3538
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis of bladder cancer cell 5637 induced by pseudolaric acid B in vitro and its mechanism.
METHODThe cell proliferation was detected by MTT assay;the cell cycle was measured by flow cytometry; the cell apoptosis was observed by flow cytometry with Annexin V-FITC/PI double staining; the expressions of survivin protein and caspase-3 protein were detected by Western blot assay.
RESULTIt showed that pseudolaric acid B remarkably induced apoptosis of 5637 cell line. Moreover, pseudolaric acid B suppressed survivin and up-regulated caspase-3 expression.
CONCLUSIONPseudolaric acid B inhibits the proliferation and induces the apoptosis of 5637 cells. The molecular mechanism of pseudolaric acid B inducing the apoptosis of 5637 cells may be associated with its action of down-regulating the expression of survivin, and up-regulating the expression of caspase-3.
