Study on electrophoretogram of Magnolia officinalis seeds from different locations.
- Author:
Xiaolin LI
1
;
Aijuan SHAO
;
Min CHEN
;
Luqi HUANG
Author Information
1. Institute of Chinese Material Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- MeSH:
Electrophoresis, Polyacrylamide Gel;
Magnolia;
chemistry;
Molecular Weight;
Plant Proteins;
analysis;
Seeds;
chemistry
- From:
China Journal of Chinese Materia Medica
2011;36(22):3075-3078
- CountryChina
- Language:Chinese
-
Abstract:
To find the identification method of seeds from different locations, we analyzed the SDS-PAGE electrophoretogram of Magnolia officinalis seeds from 5 different locations of Enshi district, Hubei province, the genuine producing area of M. officinalis, and compared band numbers, band intensity and protein molecular weight of different locations. The result showed that there were significant differences among seeds from different locations. 9 bands with the molecular weight about 80.34, 63.07, 56.48, 51.32, 40.64, 25.18, 22.63, 21.70, 18.14 kDa were involved in all seeds electrophoretograms and could be as standard bands of M. officinalis seeds. Seeds from Badong county, Jianshi county and Enshi city had their own special bands which were very different from other locations. So this electrophresis method can be applied to identify different locations of M. officinalis seeds.
