Identification of Schisandra sphenanthera and S. chinensis by random amplified polymorphic DNA sequence characterized applied region.
- Author:
Lijing CHEN
1
;
Xin QI
;
Yukun WANG
;
Li ZHANG
;
Zhifu GUO
;
Jingwei LIN
;
Yuning SONG
;
Ming ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; Molecular Sequence Data; Polymerase Chain Reaction; methods; Random Amplified Polymorphic DNA Technique; Schisandra; genetics; Sequence Analysis, DNA
- From: China Journal of Chinese Materia Medica 2011;36(22):3083-3085
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new method for the identification of Schisandra sphenanthera and S. chinensis.
METHODRandom amplified polymorphic DNA-Sequence characterized applied region (RAPD-SCAR) method was applied to screen primers.
RESULTScreening from 100 primers, only 2 random primers, which can be used to identify S. sphenanthera and S. chinensis accurately with a good reproducibility. It worked to fit them into sequence characterized applied region.
CONCLUSIONRAPD-SCAR can be used to identify S. sphenanthera and S. chinensis accurately.
