Analysis of chemical composition of sini san by ultra performance liquid chromatography-electrospray ionization-mass spectrometry.
- Author:
Rong GUO
1
;
Yan SHU
;
Li ZHANG
;
Yudan CAO
;
Anwei DING
;
Weifeng YAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Liquid; methods; Medicine, Chinese Traditional; Spectrometry, Mass, Electrospray Ionization; methods
- From: China Journal of Chinese Materia Medica 2011;36(22):3114-3118
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze and identify the chemical ingredients in Sini San by LC-MS.
METHODThese compounds were analyzed with an ACQUITY UPLC BEH C18 (2.1 mm x 100 mm, 1.7 microm) by gradient elution of methyl cyanides and 0.1% formic acid. ESI source was used and data were collected in positive and negative mode.
RESULTSeventeen compounds of Sini San were identified by direct comparison in both positive and negative modes of Q-TOF-MS, the elemental compositions analysis, the standard substance and the data of the literature.
CONCLUSIONIt is a rapid and accurate method that the composition of compound prescription of traditional Chinese medicine can be identified in terms of the separation of ultra high performance liquid chromatography, the accurate molecurlar weights measured by MS and other information, which can trace to the source of compounds in Sinisan.
