Determination of tortoside A in Ilicis pubescentis by RP-HPLC.
- Author:
Shuli LI
1
;
Yuan ZHOU
;
Pengfei TU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Glycosides; analysis; Ilex; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(22):3146-3148
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for the determination of tortoside A in Ilex pubences.
METHODKromasil-C18 (4.6 mm x 250 mm, 5 microm) column was used in HPLC with mobile phase acetonitrite-0.1% H3PO4 (17:83), the column temperature was 30 degrees C, the flow rate was 1 mL x min(-1), the detection wavelength was set at 210 nm, and inject volume was 10 microL RESULT: Tortoside A was well separated under the established conditions, the liner range of tortoside A was 26.05-521.00 microg (r = 0.999 9, n = 6), and the average recovery was 98.42%.
CONCLUSIONIt was the first time to establish the RP-HPLC method with accuracy, good reproducibility for determining the content of tortoside A in I. Pubescentis.
