Effect of single-used borneol and combining it with diazepam on content of neurotransmitter in corpus striatum of rats.
- Author:
Na ZHANG
1
;
Ping LIU
;
Xinrong HE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aspartic Acid; analysis; Blood-Brain Barrier; Bornanes; administration & dosage; pharmacology; Corpus Striatum; chemistry; drug effects; Diazepam; administration & dosage; pharmacology; Glutamic Acid; analysis; Glycine; analysis; Male; Neurotransmitter Agents; analysis; Rats; Rats, Sprague-Dawley; gamma-Aminobutyric Acid; analysis
- From: China Journal of Chinese Materia Medica 2011;36(22):3180-3183
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo research the content changes of excitatory neurotransmitter and inhibitory neurotransmitter in corpus striatum of rats after single-used borneol and combining it with diazepam in hope of comprehending the activity of borneol on central nervous system and to observe whether borneol could increase the penetration of other drugs into the brain.
METHODThe content of four amino acids neurotransmitters in corpus striatum of rats were sampled by brain microdialysis technology at different time after administration and were determined by RP-HPLC which involved pre-column derivation with orthophthaladehyde (OPA), using phosphate gradient elution and fluorescence detection to detect the content of excitatory neurotransmitter aspartate (Asp), glutamate (Glu) and inhibitory neurotransmitter glycine (Gly), gamma-aminobutyric acid (GABA) in standards and samples and carry on statistical analysis.
RESULTThe content of both Gly and GABA in corpus striatum of rats with borneol increased significantly, compared with diazepam group (P < 0.05), while Asp and Glu showed no significant difference.
CONCLUSIONBorneol can improve permeability of diazepam through BBB.
