Antioxidation properties and mechanism of action of dihydromyricetin from Ampelopsis grossedentata.
- Author:
You-sheng ZHANG
1
;
Zheng-xiang NING
;
Shu-zhen YANG
;
Hui WU
Author Information
- Publication Type:Journal Article
- MeSH: Ampelopsis; chemistry; Antioxidants; isolation & purification; pharmacology; Chelating Agents; pharmacology; Flavonols; chemistry; isolation & purification; pharmacology; Free Radical Scavengers; isolation & purification; pharmacology; Lipid Peroxidation; drug effects; Molecular Structure; Plants, Medicinal; chemistry
- From: Acta Pharmaceutica Sinica 2003;38(4):241-244
- CountryChina
- Language:English
-
Abstract:
AIMTo assess the antioxidative properties and the mechanism of action of dihydromyricetin (DMY) from Ampelopsis grossedentata.
METHODSThe antioxidative properties of DMY were measured by scavenging 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and inhibiting lipid peroxidation induced by FeSO4-edetic acid in linoleic acid. The mechanism of antioxidative properties of DMY was tested by measuring the chelating activities of DMY for Fe2+ with ultraviolet spectrum (UV) method.
RESULTSThe specific absorption of DPPH radical solution at 517 nm was reduced 73.3%-91.5% when DMY was added into the reaction solution in the concentration range from 0.01% to 0.04%. DMY was shown to greatly inhibit the increase of lipid peroxidation (LPO) values in linolei acid system catalyzed by FeSO4-edetic acid. The reaction rates (A532.min-1) of lipid peroxidation were 0.0021-0.0004 in the concentration range from 0.01% to 0.04% and the inhibition activities of DMY was found to be in a concentration-dependent manner. The mechanism of antioxidative properties of DMY was chelating Fe2+ in the Fe(2+)-dependent lipid peroxidation system.
CONCLUSIONDMY showed great antioxidative effect and would be a good natural antioxidant.
