Study on polymethacrylate nanoparticles as delivery system of antisense oligodeoxynucleotides.
- Author:
Wen-xi WANG
1
;
Hai-liang CHEN
;
Wen-quan LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Acrylic Resins; chemistry; toxicity; Animals; Drug Carriers; Drug Delivery Systems; Hemolysis; drug effects; Nanotechnology; Oligonucleotides, Antisense; administration & dosage; Particle Size; Technology, Pharmaceutical; methods
- From: Acta Pharmaceutica Sinica 2003;38(4):298-301
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the possibility of polymethacrylate nanoparticles (NP) for antisense oligodeoxynucleotides delivery system.
METHODSThe nanoparticles were prepared by evaporating ethenol solution containing Eudragit RL100 or RS100, and then mixtured with oligonucleotides. The morphology and size were investigated by a transmission electron microscope and Mastersizer particle characterization systems, and the cytotoxicity was evaluated by Trypan Blue staining and hemolysis test. The flow cytometer was used to determine the uptake of fluorescence-labelled oligodeoxynucleotides.
RESULTSThe morphology of nanoparticles showed spherical and orderly, the average diameter was about 127 nm, and almost the antisense oligodeoxynucleotides (ODN) were loaded when NP: ODN was 6.6. The uptake of ODN was significantly increased when loaded by nanoparticles, which well depended on the nanoparticles concentration. Meanwhile, slightly cytotoxicity was observed when high dose of nanoparticles was used.
CONCLUSIONThe polymethacrylate nanoparticles appeared to be a promising vehicle for gene delivery.
