Effect of Angelica sinensis polysaccharides on lymphocyte proliferation and induction of IFN-gamma.
- Author:
Jun-jie SHAN
1
;
Yi WANG
;
Shun-chun WANG
;
Di LIU
;
Zhi-bi HU
Author Information
- Publication Type:Journal Article
- MeSH: Angelica sinensis; chemistry; Animals; Cell Division; drug effects; Drugs, Chinese Herbal; isolation & purification; pharmacology; Immunity; drug effects; Interferon-gamma; biosynthesis; Lymphocytes; cytology; drug effects; immunology; Male; Mice; Mice, Inbred BALB C; Plants, Medicinal; chemistry; Polysaccharides; isolation & purification; pharmacology; Random Allocation; Spleen; cytology; Tumor Cells, Cultured
- From: Acta Pharmaceutica Sinica 2002;37(7):497-500
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of Angelica sinsensis polysaccharides on lymphocyte proliferation and induction of IFN-gamma.
METHODSAngelica sinensis polysaccharides(AP) were separated into AP-I, AP-II, AP-III and AP-IV by alcohol deposition with different concentration. The radioactivities of [3H]-TdR uptake by lymphocyte were used to determine the ability of lymphocyte. The bioactivity of IFN-gamma was measured by violet crystalline dying.
RESULTSAP-IV was found to be composed of Ara and Glu in the ratio of 0.99:6.47, the molecular weight was estimated to be 5,600. AP-I and AP-II 100 mg.kg-1 i.p. were found to significantly augment mice splenocyte proliferation, release IFN-gamma and increase IFN-gamma bioactivity. 50 micrograms.mL-1 AP-I, AP-II and AP-III were shown to enhance the proliferative response of the mouse spleen lymphocytes in vitro.
CONCLUSIONAP-I and AP-II showed higher immunoactivity than AP-III, AP-IV had no effect.
